TABLE 3.
Characteristics of reconstructed Amdr mutants of E. coli MG1655 strain DA5438 compared to corresponding laboratory-selected mutants
| Strain | Amd resistance-encoding mutation(s) | MIC (mg/liter) | Relative growth rate |
|---|---|---|---|
| DA5438 | None | 0.125 | 1 |
| DA18488 | thrS (E519G) | 48 | 0.31 |
| DA29703 | thrS (E519G)a | 32 | 0.76 |
| DA18480 | aspS (P191S) | 16 | 0.54 |
| DA28434 | aspS (P191S)a | 24 | 0.61 |
| DA18497 | gltX (D180G) | 32 | 0.33 |
| DA30120 | gltX (D180G)a | 24 | 0.36 |
| DA18467 | ubiE (delb bp 346–349 → FSc) | 12 | 0.46 |
| DA28436 | ubiE (del bp 346–349 → FS)a | 12 | 0.54 |
| DA18483 | ubiX (del bp 246 → FS) | 32 | 0.37 |
| DA29707 | ubiX (del bp 246 → FS)a | 32 | 0.40 |
| DA18491 | ispA (IS1 insertion) | 12 | 0.35 |
| DA30122 | ispA (IS1 insertion)a | 16 | 0.33 |
| DA18477 | rplL (promoter −10 and duplication) | 8 | 0.62 |
| DA29710 | rplL (promoter −10)a | 48 | 0.34 |
| DA18481 | ppa (D11A) | 96 | 0.48 |
| DA28432 | ppa (D11A)a | 96 | 0.43 |
| DA18482 | rpoB (H447L), araB (I450L) | 32 | 0.56 |
| DA29712 | rpoB (H447L)a | 48 | 0.61 |
| DA18493 | spoT (S305P) | 24 | 0.75 |
| DA28438 | spoT (S305P)a | 32 | 0.76 |
| DA18489 | mrdA (D389G), trxB (G37D) | 64 | 0.30 |
| DA18490 | mrdA (D389G) | 16 | 0.41 |
| DA29705 | mrdA (D389G)a | 16 | 0.51 |
| DA29715 | trxB (G37D)a | 0.064 | 0.92 |
| DA29717 | mrdA (D389G),a trxB (G37D)a | 64 | 0.29 |
a
Reconstructed mutant.
b
del, deletion.
c
FS, frame shift.