TABLE 4.
Putative Amd resistance-encoding mutations in the clinical isolates used in this study
| Strain | MIC (mg/liter) | Mutation(s) in known Amd resistance-encoding gene(s)a | cysB mutation |
|---|---|---|---|
| DA14704 | 32 | rcsC (T714N), yrfF (R50H), alaS (A515T), mreC (Q324P, delb ntc 975–1035) | V12G |
| DA14709 | 4 | rcsB (K192T), rcsD (A254T), rcsF (P26S), gltX (D301N), aspS (K347N), spoT (T350I), rpoB (G544S) | IS1 insertion |
| DA14710 | 64 | rcsF (P48S), gltX (S65N, T287A), alaS (D712E) | G241E |
| DA14712 | 32 | rcsF (P48S), gltX (S65N, T287A), argS (Y200C), alaS (D712E) | FSd after aae 160 |
| DA14713 | 64 | rcsD (P252Q), yrfF (D307N), aspS (S580G), slt (S2266T, P544L) | Q103stop |
| DA14715 | 16 | thrS (P7H), ftsQ (E262G), ftsZ (V331A) | del nt 278–309 |
| DA14718 | 32 | FS after aa 161 | |
| DA14719 | 16 | crp (T8A, S17F) | FS after aa 87 |
| DA24678 | 48 | rcsD (P490L), rpoB (E625A) | FS after aa 161 |
| DA24686 | 32 | rcsD (P252Q), yrfF (D307N), argS (E326K), mreC (S69L, A327T, P352S), cyaA (Q655L, L693P), slt (T142I) | K76stop |
| DA24690 | 32 | rcsD (P490L), rpoB (E625A) | Q4stop |
| DA24692 | 48 | ubiE (S5L), cyaA (del nt 1428–1434), slt (G58R, G540S), galE (E67K) | FS after aa 242 |
a
Not found in reference genomes.
b
del, deletion.
c
nt, nucleotide.
d
FS, frame shift.
e
aa, amino acid.