TABLE 1:

Comparative mass spectrometry (MS/MS) analysis of the DHC-containing fractions of wild-type and FAP206-KO axonemes.

DHC name	Gene ID	Protein accession number	Peptide matched number
		CU428	FAP206-KO
DYH5	TTHERM_00486600	gi|118401102|ref|xp_001032872.1|	1296	1324
DYH4	TTHERM_00499300	gi|118378024|ref|xp_001022188.1|	1216	1265
DYH3	TTHERM_01276420	gi|118394992|ref|xp_001029853.1|	1133	1115
DYH7	TTHERM_00912290	gi|118374012|ref|xp_001020198.1|	551	600
DYH6	TTHERM_00688470	gi|118387693|ref|xp_001026949.1|	581	588
DYH15	TTHERM_00433800	gi|118356293|ref|xp_001011405.1|	498	492
DYH16	TTHERM_00558640	gi|118378501|ref|xp_001022426.1|	280	294
DYH22	TTHERM_00565600	gi|118377765|ref|xp_001022060.1|	315	263
DYH11	TTHERM_00252430	gi|229595213|ref|xp_001019094.2|	232	216
DYH19	TTHERM_01027670	gi|118396733|ref|xp_001030704.1|	136	152
DYH24	TTHERM_00193520	gi|118367791|ref|xp_001017105.1|	141	128
DYH25	TTHERM_00774810	gi|118376063|ref|xp_001021214.1|	233	0
DYH14	TTHERM_00492830	gi|118380021|ref|xp_001023175.1|	65	70
DYH12	TTHERM_00919540	gi|118389527|ref|xp_001027847.1|	53	0
DYH20	TTHERM_00821980	gi|118398395|ref|xp_001031526.1|	25	15
DYH9	TTHERM_00947430	gi|118397291|ref|xp_001030979.1|	11	11
DYH8	TTHERM_00531870	gi|118382309|ref|xp_001024312.1|	7	13
DYH23	TTHERM_00355100	gi|118354291|ref|xp_001010408.1|	8	8
DYH10	TTHERM_00420340	gi|118401939|ref|xp_001033289.1|	11	0
DYH2	TTHERM_00558310	gi|118378437|ref|xp_001022394.1|	6	3
DYH1	TTHERM_00046310	gi|118363224|ref|xp_001014626.1|	1	0

Among the 25 predicted DHCs (based on the genome of T. thermophila (Eisen et al., 2006), we detected peptides that correspond to 21 DHCs in the wild-type axoneme. The DHCs that were predicted but not detected are DYH13, DYH17, DYH18, and DYH21. Of importance, DYH25, DYH12, and DYH10 are detectable in the wild-type axoneme but missing in the FAP206 axoneme. Thus DYH25, DYH12, and possibly DYH10 (the case of DYH10 is less strong due to its low abundance in the wild-type sample) collectively constitute dynein c of Tetrahymena.