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. 2015 Feb 15;26(4):762–768. doi: 10.1091/mbc.E14-07-1186

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© 2015 Gnanasundram and Koš. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Comparison of depletion of various proteins in TetO7 and Tet07-Ubi-Leu strains. Growth curves (left) and Western blot analysis (middle and right) of strains depleted of essential proteins Dbp4p (A), Bfr2p (B) Erg1 (C), Sec62 (D), Prp19 (E), and Rrn3 (F) using different depletion cassettes. Samples were collected at the indicated time points following the addition of doxycycline. The individual proteins were visualized using antibodies against the HA tag and actin. The quantification of the blots, normalized to actin levels, is shown on the right; a mean of two independent experiments is shown. For growth curves (left charts), the mean of three independent experiments is plotted; vertical y-axes are in logarithmic scale.