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. 2015 Feb 12;6:20. doi: 10.3389/fneur.2015.00020

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Copyright © 2015 Miller, Shah, Aperi, Budde, Pintar, Tarima, Kurpad, Stemper and Glavaski-Joksimovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preservation of OHCs’ structural organization during culturing period. (A) Light micrograph of an acutely dissected OHC. (B) Same OHC as in (A) demonstrates well preserved CA1, CA3, and DG hippocampal regions at 8 DIV. Higher magnification of CA1 (C) and DG (D) regions of cresyl violet-stained OHC at 8 DIV also illustrate intact hippocampal cytoarchitecture. Serial imaging of PI-stained OHC at 1 (E), 5 (F), and 8 (G) DIV demonstrates recovery of slice from procedure-related cellular degeneration. Scale bars (A,B) 500 μm; (C,D) 50 μm; (E–G) 500 μm.