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. 2015 Feb 12;6:20. doi: 10.3389/fneur.2015.00020

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Copyright © 2015 Miller, Shah, Aperi, Budde, Pintar, Tarima, Kurpad, Stemper and Glavaski-Joksimovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Live microglia imaging in OHCs following blast exposure. Microglial cells in OHCs were labeled with IB4 (green) and images of CA1 region were captured at 4 h (A–C) and 24 h (D–F) following sham (A,D) or blast injury (B,C,E,F). OHCs maintained low level of microglial activation and PI staining (red) at 4 h (A) and 24 h (D) following sham injury. Dead microglial cells that were co-labeled with IB4 and PI (arrows) were observed in low- (B) and high- (C) blast group at 4 h post-injury. Even more prominent microglial death was detected in low- (E) and high- (F) blasted OHCs at 24 h post-injury. Activation-induced change in microglia morphology from ramified to rounded was also observed in blasted OHCs (B,C,E,F). Scale bars 50 μm.