Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 26;3:13. doi: 10.1186/2045-9769-3-13

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ahearne et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

P2 and P4 ASCs cultured from two different donors in chemically defined differentiation media after 21 days. (A) Adipogenic assay stained using oil red O and quantified by measuring the percentage of surface area stained; (B) osteogenic assay stained using alizarin red and quantified by measuring the light absorbance at 405 nm; (C) chondrogenic pellet assay stained using alcian blue and nuclear fast red and quantified using biochemical assays for DNA and GAG. n = 3, ● represents a significant difference between FGF and the equivalent non-FGF-supplemented media; o represents a significant difference between P2 and P4 cells; *significant difference between donors, p < 0.05; scalebar = 20 μm for A and B and 100 μm for C, representative images display the results from P2 donor 1.