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. 2014 Dec 5;15(1):946. doi: 10.1186/1471-2164-15-946

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© Allen et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Testing the functional importance of the CydX C-terminal amino acids. (A) Alignment of the E. coli CydX protein sequence along with six mutant sequences containing mutated C-terminal amino acid sequences. (B) Assay of CydX function was conducted using a zone assay testing the sensitivity to β-mercaptoethanol. Sensitivity was measured using zones of inhibition, and the diameter of the zone after addition of 10 μL of 12 M β-mercaptoethanol to a plate of bacteria is shown. The average and standard deviation of zone sizes was calculated from at least three replicate plates. Alignments were generated using the program MUSCLE [57].