Table 3.
Residues of UNC-60A and UNC-60B showing effective correlation time (τe) and chemical exchange (Rex) from Modelfree analysis and relaxation dispersion analysis
| Modelfree | Residues of UNC-60A | Residues of UNC-60B |
|---|---|---|
| Residues in effective correlation time (τe) | G4-V7 (N-terminal and β1), Q12 (α1), G21, K23-E24 (loop after α1), G52-D53 (insert after β3), R93-G97 (F-loop), K117(α3), T135(loop after α3), S150 (loop before C-terminal helix), and Y162-H165 (C-terminal region) | G4-K6 (N-terminal and β1), N34 (loop between β2 and β3), V61(α2), E66 (loop after α2), T77-Q79 (β4), Q81-N90, V92 (F-loop), C98, D100 (loop after β5), S148, and Q150-I152 (C-terminal) |
| Residues in chemical exchange(Rex) | D10-F15, K17-S19 (α1), S59-A61(second helical turn of extra region), F63-K65, V67-T74 (α2 and loop after α2), C80 (β4), C110 (loop after β5), K130 (loop after α3), V143 (loop after β6), and H165 (C-terminal) | K12-N13, Y15, L17-L18 (α1), K21(loop after α1), F29 (β2), V43-G44 (β3), Y50-A51, F53-V54, M57-K58, V61(α2), V74 (β4), C98, D100 (loop after β5), R105 (α3), G122-L123, L126 (loop after α3), N130 (β6), D136 (helical turn), and S144 (α4) |
| Relaxation Dispersion | Residues of UNC-60A | Residues of UNC-60B |
| Residues in chemical exchange(Rex) | Q12-S14, K17, S19, E20 (α1), E40, T44, T51, G52, D54, D57 (insert after β3), A61, K65, V67-V70 (α2 ), D75-L77 (loop after α2), G95-T98, K100-D102 (F-loop), C110, D112, G113 (β5-α3 loop), K119, Y122, S125, T131 (α3), L133, T135 (α3-β6 loop), D145, E146, E148 (helical turn), K152-L154, N156, L158, K161 (C-terminal) | S10, K12-A14, H19 (α1), N34-D35 (loop between β2-β3), E52-E55, M57, K59 (α2), E62, G64, E66 (loop after α2), R105, L109, Y110, L117, K118, L121 (α3), L123, L126 (α3-β6 loop), D138 (loop after β6), V142-S144, L146 (C-terminal) |