Fig. 1.

An ELISPOT assay for identifying regions containing potential HPV-specific CD4 T-cell epitopes. One hundred thousand CD4 T-cells stimulated in vitro with autologous dendritic cells pulsed with HPV 16 E6 and E7 proteins were tested using overlapping peptides [9]. Each peptide pool contains three overlapping 15-mer peptides. “*” indicates regions with positive CD4 T-cell responses defined by having spot forming units ≥ 2 times those in the media only control wells. The bars represent standard error of means.