Table 1.
Receptor tyrosine kinases and downstream adaptor molecules identified from phosphoproteomics
| pY site | Sequence | Autocatalysis site (Y/N) |
Fold Change |
P value | |
|---|---|---|---|---|---|
| H2286 | |||||
| EGFR | pY1092 | YSSDPTGALTEDSIDDTFLPVPEyINQSVPK | Y | 2.37 | 0.0352 |
| pS1166/pY1172 | GSHQIsLDNPDyQQDFFPK | N/Y | 2.46 | 0.0148 | |
| pY1110 | RPAGSVQNPVyHNQPLNPAPSR | Y | 2.22 | 0.0007 | |
| pY1197 | GSTAENAEyLR | Y | 1.95 | 0.0272 | |
| GAB1 | pY259 | APSASVDSSLyNLPR | 2.27 | 0.04 | |
| pY373 | TASDTDSSyCIPTAGMSPSR | 2.76 | 0.00 | ||
| pY406 | DASSQDCyDIPR | 2.80 | 0.01 | ||
| pY659 | SSGSGSSVADERVDyVVVDQQK | 1.94 | 0.01 | ||
| ERBB2 | pY1023 | SLLEDDDMGDLVDAEEyLVPQQGFFCPDPAPGAGGMVHHR | N | 3.18 | 0.0803 |
| pY877 | LLDIDETEyHADGGKVPIK | N | −8.20 | 0.0155 | |
| IGF-1R | pY1161/pY1165 | DIyETDyYRK | Y/N | 2.68 | 0.0016 |
| pY1161 | DIyETDYYR | Y | 3.20 | 0.0012 | |
| pY1165 | DIYETDyYR | N | 2.58 | 0.0017 | |
| IRS2 | pY598 | QRPVPQPSSASLDEyTLMR | 2.22 | 0.0035 | |
| pY675 | SDDyMPMSPASVSAPK | 2.00 | 0.0096 | ||
| pY742 | ASSPAESSPEDSGyMR | 3.97 | 0.0112 | ||
| MET | pY1234/5 | DMYDKEyySVHNK | Y/Y | 1.87 | 0.0419 |
| AXL | pY698 | KIyNGDYYR | N | 2.87 | 0.0046 |
| HCC366 | |||||
| EGFR | pY1092 | YSSDPTGALTEDSIDDTFLPVPEyINQSVPK | Y | 2.03 | 0.0003 |
| pS1166/pY1172 | GSHQIsLDNPDyQQDFFPK | N/Y | 1.77 | 0.0165 | |
| pY869 | LLGAEEKEyHAEGGKVPIK | N | −11.91 | 0.0066 | |
| GAB1 | pY659 | SSGSGSSVADERVDyVVVDQQK | 1.52 | 0.0159 | |
| ERBB2 | pY1023 | SLLEDDDMGDLVDAEEyLVPQQGFFCPDPAPGAGGMVHHR | N | 1.63 | 0.0224 |
| pY1248 | GAPPSTFKGTPTAENPEyLGLDVPV | Y | 2.13 | 0.0128 | |
| pY877 | LLDIDETEyHADGGKVPIK | N | −63.28 | 0.0002 | |
| IGF-1R | pY1161 | DIyETDYYRK | Y | 1.68 | 0.0166 |
| IRS2 | pY598 | QRPVPQPSSASLDEyTLMR | 1.56 | 0.0404 | |
| pY653 | SSSSNLGADDGyMPMTPGA | 1.67 | 0.0078 | ||
| pY766 | LLPNGDyLNVSPSDAVTTG | 1.50 | 0.0478 | ||
| pY978 | SPLSDyMNLDFSSPK | 1.56 | 0.0317 | ||
| MET | pS988/pY1003 | sVSPTTEMVSNESVDyR | N/N | 1.50 | 0.0141 |
| pT992/pY1003 | SVSPtTEMVSNESVDyR | N/N | 2.30 | 0.0020 | |
| AXL | pY702/pY703 | IYNGDyyRQGR | N/Y | 1.97 | 0.0039 |
| pY759 | GQTPYPGVENSEIyDYLR | N | −1.79 | 0.0001 | |
Fold change indicates the intensity of extracted ion chromatogram (EIC) after dasatinib treatment. Phosphorylated amino acids are shown in lowercase letters.