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. Author manuscript; available in PMC: 2015 Feb 12.
Published in final edited form as: Immunity. 2013 Dec 12;39(6):1108–1120. doi: 10.1016/j.immuni.2013.11.009

Fig 1. S. Typhimurium fails to elicit MC activation and subsequent neutrophil recruitment and bacterial clearance in vivo.

Fig 1

(A, B, top) Residual bacterial counts (CFUs) in the peritoneal cavity of wild-type (WT) or MC-deficient mice 24 h following intraperitoneal (i.p.) infection with 5×105 CFU S. Typhimurium or 1×107 CFU E. coli. (A, B, bottom) Myeloperoxidase assay of peritoneal lavage of WT or MC-deficient mice 5 h post-infection with 1×107 CFU S. Typhimurium or E. coli J96 (C) Morphologic appearance of MCs in the peritoneum (top) and mesentery (bottom) of WT mice 3 h after i.p infection of 1×108 CFU S. Typhimurium or E. coli J96. Insets represent 60X magnification of MCs in the mesentery. Images represent 3 independent experiments. Top panels and inset scale: 20 µm. Bottom panels scale: 100 µm. n=4. (D) Granulated MC numbers in peritoneal lavages (top) or mesentery whole mounts (bottom) were quantified by counting the number of partially and fully granulated MCs/field (n=3–5; 5 random chosen fields). Wholly degranulated MCs could not be detected. Mean ± SEM, *p<0.05, **P<0.01, N.S., not significant.