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. Author manuscript; available in PMC: 2015 Feb 12.
Published in final edited form as: Nat Protoc. 2014 Sep 18;9(10):2411–2424. doi: 10.1038/nprot.2014.154

Figure 2. Major elements of procedure.

Figure 2

(a) The target gene(s) abundantly expressed in a cell type of interest are identified and confirmed using RT-PCR in Steps 1–5. (b) MBs are designed by identifying unique target sequences that are accessible, and choosing a stem region and an appropriate dye-quencher pair. (c) MBs are tested in solution to check the quality of MB synthesis and in positive/negative control cells to ensure that a high fluoresce signal is generated only in the specific cell type of interest. (d) Stem cells are differentiated into target cells (e.g., CMs), MBs are delivered into cells in the entire culture of mixed cell population, and MB positive cells are isolated using FACS. (e) MB-positive cells are characterized to confirm that they are the right cell type with high purity using RT-PCR, immunocytochemistry, and electrophysiology (for CM).