Fig. 5.

Gene ontology analysis of up- and down-regulated proteins. (a) Protein functional classification. 383 proteins that were found differentially expressed across all three donors could be classified into 25 categories, of which the top four are nucleic acid binding proteins (17%), hydrolase (11.3%), enzyme modulator (10.4%), and transferase (10.2%). (b) Molecular function. More than 70% of IL-2 regulated proteins have molecular function related to catalytic activities (41%) and binding (34%). (c) Biological process. IL-2 regulated proteins were found to be involved in 16 biological processes, of which the top three categories are: metabolic process (30.4%), cell process (15.9%) and transport (11.4%). Gene ontology analysis was performed in PANTHER database (http://www.pantherdb.org/).