Figure 2.

Loss of ikbkap resulted in Hirschsprung disease-like phenotype. A: Control embryo showed normal enteric nervous system (ENS) development with enteric neurons present along the whole intestine (reddish-brown); B: Morphant displayed complete absence of enteric neuron in distal intestine (asterisk); C: Morphant showed milder phenotype of reduced number of enteric neurons. Magnification × 200 and × 400; D: Summary of aganglionosis observed in morphants (n = 110) and control (n = 46). The difference was statistically significant (P < 0.01) by χ² test; E: Summary on the number of enteric neurons in morphants (n = 17) and control (n = 17). The difference was statistically significant (38.8 ± 9.9 vs 50.2 ± 17.3, P < 0.05) by Student’s t test.