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Loss of ikbkap caused phox2bb down-regulation. A: Qualitative real-time polymerase chain reaction for the expressions ret, phox2bb and sox10. elfa served as internal control; B: Quantitative-polymerase chain reaction for ret and phox2bb. Down-regulation of phox2bb was statistically significant (0.51 ± 0.15 vs 1.00 ± 0, P < 0.05) by Student’s t test.
