Fig. 1.

M.abs increased HO-1 expression. TPA-differentiated THP-1 cells were infected with M.abs for 1 h, washed and incubated in media containing gentamicin until harvested at different time points. M.abs cfu were determined and cells were prepared for immunoblotting as described in Materials and methods. (A) M.abs showed robust intracellular growth, as shown by the increase in CFU at days 0, and 1 post-infection. (B) Proteins were run and HO-1 visualized by immunoblotting. Results showed induction of HO-1 after infection. (C) Densitometric analysis of HO-1 bands in (B). M.abs infection resulted in a significant increase in expression of HO-1 (n=4, *indicates p<0.001).