Fig. 7.

M.abs infection increases THP-1 cell GSH turnover, but not MnSOD or catalase protein expression or activity. TPA-differentiated THP-1 cells were infected with M.abs for 1 h, washed and cultured in media until collected at different time points. Immunoblotting and enzyme activity were determined as described in materials and methods. (A) No difference in catalase or MnSOD protein (Panel A), SOD activity (Panel B, n=3, p>0.05) or catalase activity (Panel C, n=3, p>0.05) was observed between M.abs-infected and non-infected THP-1 cells at different points. (Panel D) Cells were then collected and studied for reduced glutathione (GSH), and oxidized glutathione (GSSG) levels as described in materials and methods. M.abs significantly decreased the ratio of GSH/GSSG, which reflects glutathione turnover (n=3, p<0.05). Data counts in B–D were normalized to uninfected controls.