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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Jan 9;431(2):215–220. doi: 10.1016/j.bbrc.2012.12.132

Figure 3. Polyclonal rabbit serum raised against Antigen 1 is able to block AniA nitrite reductase activity of N. gonorrhoeae 1291 cells.

Figure 3

Bars represent AniA activity expressed as the mean reaction rate (nmoles of nitrite reduced/min/OD600) from triplicate samples over the course of a nitrite utilisation assay. Samples include 1291 cells pre-incubated with 1:10 baby rabbit serum (BRS) (1291 + BRS), 1291 cells pre-incubated with 1:10 pre-immune rabbit serum (1291 + pre-immune), 1291 cells pre-incubated with 1:10 polyclonal rabbit serum raised against Antigen 1 (1291 + Antigen 1 final) and 1291 aniA::kan cells pre-incubated with 1: 10 BRS (1291 aniA::kan + BRS). Error bars represent +1 standard deviation from the mean. The reaction rate for cells pre-incubated with polyclonal rabbit serum raised against Antigen 1 was significantly lower compared to cells pre-incubated with either BRS (** p =0.0443) or with pre-immune rabbit serum (* p = 0.0144).