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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Biomaterials. 2015 Jan 13;45:56–63. doi: 10.1016/j.biomaterials.2014.12.021

Figure 3. HGF-f binds to heparin and ECM hydrogels.

Figure 3

(A) HGF-f was loaded onto a heparin affinity column and eluted using a NaCl gradient. The chromatogram corresponds to protein elution from the column, as measured by absorption at 280 nm. HGF-f elutes as a single peak in phosphate buffered saline with an additional 1.1 M NaCl, indicating strong binding to heparin. (B) Relatively rapid diffusion of HGF-f is observed from collagen hydrogels (open circles), while slow release is seen from ECM hydrogels (solid circles). Nearly complete release of HGF-f from collagen hydrogels is seen post-collagenase treatment, while treatment with 1.5 M NaCl solution is needed to fully dissociate HGF-f from the ECM hydrogels. Data is mean ± SEM.