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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Immunol Methods. 2014 Jul 26;413:1–11. doi: 10.1016/j.jim.2014.07.009

Fig. 2.

Fig. 2

Effect of anticoagulant on baseline and GM-CSF-stimulated CD11bSurface. Fresh blood was collected from healthy people into ethylenediaminetetraacetic acid (EDTA) – or sodium heparin-containing phlebotomy tubes and the mean fluorescence intensity (MFI) of CD11b on CD16Hi-gated neutrophils (CD11bSurface) was measured after stimulation with 10 ng/ml GM-CSF using the CD11b-SI test as described in the Methods. Bars represent the mean ± SD for n = 3 per condition. Comparisons were made by ANOVA with Dunnett's test; *** = P<0.001.