Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Immunol Methods. 2014 Jul 26;413:1–11. doi: 10.1016/j.jim.2014.07.009

Fig. 5.

Fig. 5

Effects of temperature of blood after phlebotomy and delay in analysis on change in CD11bSurface. A. Effect of temperature on CD11bSurface expression levels. Fresh, heparinized blood was collected and maintained at the indicated temperatures and then CD11b expression levels were evaluated as described in the Methods. B. Effect of a short delay prior to analysis on the change in CD11bSurface stimulated with 10ng/mL of GM-CSF. Fresh, heparinized blood was kept at 25°C for various times before evaluation by the change in CD11bSurface as described in the Methods. Symbols represent the mean ± SD for n = 3 per point. Comparisons to the corresponding value at zero hours were made by ANOVA with Dunnett's test; * = P<0.05, ** = P<0.01, *** = P<0.001.