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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Immunol Methods. 2014 Jul 26;413:1–11. doi: 10.1016/j.jim.2014.07.009

Fig. 8.

Fig. 8

Detection of impaired GM-CSF signaling in human blood specimens. Fresh, heparinized blood was collected from healthy people (n = 22; gray bars) or patients with autoimmune PAP (n = 5, closed bars) and percent increase of CD11bSurface over baseline as described in the Methods except that different GM-CSF concentrations were used for stimulation (indicated). Comparisons were made by the Mann-Whitney rank sum test with post-hoc analysis using Dunnett's test. comparison of corresponding values for healthy people or PAP patients to the un-stimulated control (no GM-CSF) are indicated; * = P<0.05. Comparison of values for healthy people and PAP patients at each level of GM-CSF stimulation are also indicated; †† = P<0.01, ††† = P<0.001.