Table 1. Discordance with biopsy: False positives and false negatives in Fig. 5A and corresponding clinical notes.

Among the five most significant outliers (elevated cfdDNA) in the group of samples with biopsy grade 0, four samples corresponded to patients diagnosed with mild rejection (grade 1R) within 6 weeks of the discordant reading. Among the five greatest outliers in the group of mild rejections (grade 1R), four samples corresponded to patients who were diagnosed within 2 months with a moderate or severe ACR (≥2R) or AMR event. Last, among the samples for which a low cfdDNA level was recorded, although the biopsy grade indicated a moderate rejection (2R), three patients were asymptomatic and had normal allograft function.

Type	Patient	Months after transplant	Donor fraction corrected (%)	Endomyocardial biopsy grade	Clinical notes
False positive	A30	2.5	1.21	0	Mild rejection at month 4
False positive	A28	3	0.68	0	Mild rejection at month 4
False positive	P4	1	0.45	0
False positive	P13	1	0.4	0	Mild rejection at month 2
False positive	A16	0.75	0.4	0	Mild rejection at month 1.25
False positive	A28	8	5	1R/1B	Moderate rejection at month 9
False positive	P13	6.5	4.7	1R/1A	AMR at month 5
False positive	A25	16	2.4	1R/1A
False positive	P20	10	2.8	1R/1A	Severe rejection at month 12
False positive	P13	3	1.67	1R/1A	Moderate rejection at month 4
False negative	A34	4	−0.06	2R/3A	Normal allograft function
False negative	A6	8	−0.02	2R/3A	Normal allograft function
False negative	A4	3	0.03	2R/3A	Chagas disease reactivation
False negative	A40	1	0.04	2R/3A
False negative	A10	3	0.06	2R/3A	Normal allograft function