Fig. 10.

Neuromodulation of Kv1.3 by IR kinase is activity dependent. A: olfactory bulbs from control or naris-occluded animals were harvested at P20 and placed into primary cell culture at equivalent neuronal cell density. Plot of the mean reactive neurons that were immunolabeled for IR kinase or Kv1.3 channel and counted in a double-blind experiment across 10 to 20 microcopic fields. *Significantly different Student’s t-test. B: voltage-clamp recordings in the whole-cell configuration for the primary cultures described in A. Neurons were held at –90 mV and stepped to a single depolarizing potential of +40 mV before (Control) and after (Insulin) 20-min bath application of 0.1 μg/ml insulin. For statistical comparisons, see Table 2.