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. 2015 Feb 7;11:22. doi: 10.1186/s12917-015-0331-y

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© Zhang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Ectopic expression of Zhangfei causes decreased ability of canine osteosarcoma cells to repair a scratch wound. (A) Scratch wounds were made in 100% confluent cultures of D–17 or Abrams cells mock-infected or infected with Adeno-ZF or Adeno-LacZ. Phase contrast images were taken at 0, 4, 8, 12, and 24 hours after infection from identical regions. (B) The wound size relative to the starting wound size was measured at each time point after infection in three independent experiments and expressed as a percentage reduction in wound size + standard deviation. Significance of differences of means (*P < 0.05, **P < 0.01) were determined using ANOVA.