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. 2014 Dec 13;107(2):dju407. doi: 10.1093/jnci/dju407

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Figure 3. — AZD1208 treatment effect on phospho-Bad staining in prostate tissue recombinant grafts. A) Vehicle-treated and (B) AZD1208-treated tissue recombinant grafts with adjacent sections stained by hematoxylin and eosin (H&E), yellow fluorescent protein (YFP) (anti-GFP antibody), and phospho-Bad-S112 antibody. Abnormal gland foci are indicated by arrows. Phospho-Bad(S112) expression is reduced within the abnormal gland foci in AZD1208-treated specimens compared with vehicle-treated samples, indicating that PIM kinase function is inhibited in the prostate tissue. Scale bars = 0.1mm (short bar) and 0.05mm (long bar). H&E = hematoxylin and eosin; GFP = green fluorescent protein.