Figure 5.

AZD1208 effects on sensitivity of prostate cancer cells to radiation. A) Immunoblots demonstrate induction of Pim1 (44kD and 33 kD isoforms) by radiation and hypoxia in Myc-CaP cells. B) Clonogenic assay demonstrates that AZD1208 acts as a radiation sensitizer by decreasing the surviving fraction when compared with radiation with vehicle (replicates n = 4 per radiation dose). *Student’s t test P < .05. All statistical tests were two-sided. C) Increased Pim1 expression in Myc-CaP tumors treated with radiation and hypoxia. Scale bar = 0.05mm.