Figure 5.
Microglial activation in Cln1R151X mice. Immunohistochemical staining for CD68 reveal microglial reactivity, which exhibit macrophage-like morphology, in the cortex, thalamus and hippocampus of Cln1R151X mice. Quantitative image analysis reveals the significantly increased expression level of CD68 within the cortex, thalamus and hippocampus of Cln1R151X mice compared with age- and sex-matched wild-type controls. Statistical significance was determined by unpaired t-test with Welch's correction. Data are plotted as the mean sum immunoreactivity of each genotype as a fold-change compared with wild type (30 fields per animal; 3 animals per genotype) ± SEM.