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. 2014 Aug 28;24(1):230–242. doi: 10.1093/hmg/ddu441

Figure 3.

Figure 3.

Expression of WT and mutant IFT172 constructs in mIMCD3 cells. (A) Anti-V5 staining of IFT172 WT and mutant proteins (red) in mIMCD3 cells expressing somatostatin receptor 3 (SSTR3)—GFP fusion protein (the size bar represents 10 µm). Cell nuclei were visualized by Hoechst staining. (B) Quantification of cilia length in mIMCD3 cells transfected with a control pCAG-enhanced green fluorescent protein (EGFP) vector, WT or mutant constructs. The boxes represent 25–75% quintiles and the whiskers represent the 5–95% quintiles. The p.L275P mutant did not locate to the cilium and therefore this condition was excluded from the cilia length quantification (**P< 0.01, ***P < 0.0001). (C) Real-time quantitative PCR of mouse and human IFT172 transcripts in the transfected and untransfected mIMCD3 cells. The untransfected mIMCD3 cells condition was used as a calibrator sample and mIMCD3 cells transfected with the empty pCAG-EGFP served as a control.