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. 2015 Jan 29;2015:235265. doi: 10.1155/2015/235265

Figure 3.

Figure 3

Involvement of PI3K/Akt signaling in the protective effects of icariin against Aβ 25–35-induced cytotoxicity in PC12 cells. After 1 h pretreatment with the PI3K inhibitor LY294002 (50 μM), PC12 cells were treated with 20 μM icariin for 1 h, followed by Aβ 25–35 treatment for 24 h. Cell viability and apoptosis were determined by MTT assay and Annexin V/PI double staining, respectively. (a) LY294002 inhibited the icariin-mediated increase in viability in Aβ 25–35-treated cells. (b) LY294002 inhibited the icariin-mediated decrease in apoptosis in Aβ 25–35-treated cells. (c) (A) Untreated control, (B) Aβ 25–35 treatment, (C) icariin treatment, (D) LY294002 treatment, and (E) icariin + LY294002. Results are presented as mean ± S.D. of triplicate independent experiments. ## P < 0.01 versus untreated control; ** P < 0.01 versus Aβ 25–35 treatment; $$ P < 0.01 versus icariin treatment.