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. 2015 Feb 3;13:7. doi: 10.1186/s12964-015-0087-9

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© Gupta et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PI3K activity associated with CNGA1 is independent of light and IR activation. Solubilized ROS from either dark or light-adapted mice (A) or IR wild-type (C) or IR knockout (C) mice were immunoprecipitated with anti-CNGA1 antibody or non-immune IgG. PI3K activity was measured using PI-4,5-P₂ and [γ³²P]ATP as substrates. The radioactive spots of PI-3,4,5-P₃ were scraped from the TLC plate and counted (B, D). Data are mean ± SD (n = 3).