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. 2015 Jan 23;16(1):22. doi: 10.1186/s12864-014-1202-9

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© Chang et al.; licensee Biomed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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RT-qPCR validation of the differentially regulated calcium channel genes at the epithelium (biological repeats ≥ 3). Cosine similarity-identified A) CACNA1D and B) ORAI1 were tested with different primer sets. Other high voltage-gated calcium channels C) CACNA1H D) CACNA2D1 and E) CACNA1G showed distinct expression patterns between feather and scale epithelium. *represents p-values < 0.05 for the bracketed comparisons.