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. 2015 Jan 23;16(1):22. doi: 10.1186/s12864-014-1202-9

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© Chang et al.; licensee Biomed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ChIP-qPCR of the potential enhancer regions that may be associated with CACNA1D gene activities. A) Mapped ChIP-seq reads on and near CACNA1D; ChIP-seq on histone markers H3K4me1, H3K27ac, and H3K27me3 was performed for undifferentiated feather epithelium from embryonic day 7 (E7fe) and undifferentiated scale epithelium from embryonic day 9 (E9se). The positions chosen for ChIP-qPCR amplifications (black vertical bars) were designed based on the most differentially marked regions identified by MACS peak calling analysis. B-D) ChIP-qPCR results for enhancer-associated histone markers: H3K4me1, H3K27ac, and H3K27me3, respectively. Results are arranged in sequence from left to right, matching the sites for ChIP-qPCR amplification in A). “US” denotes primer set targeting the region upstream of the gene; “IN” denotes primer set targeting the intron of the gene. *represents p-values < 0.05 for the bracketed comparisons.