Fig. 4.

Genetic interactions between histone chaperones ASF1 and FACT. a Cell patches for the fivefold spot dilution assay were grown for the indicated strains on the YPDA plates for 2 days at the indicated temperatures. b ChIPs using an antibody raised against total histone H3 were performed in the tet-SPT16 and tet-SPT16/ΔASF1 strains in the absence (No Dox) or presence (+Dox) of doxycycline. For each panel, the time course of heat shock is shown—(0 to 64 min) after switch of temperature from 30 to 39 °C. Data were obtained and represented as described for Fig. 1b