Fig. 3.

Aβ increases in intracellular calcium concentration in primary human neurons. Intracellular calcium levels measured from human neurons, at consecutive days of continuous exposure to Aβ. a Cells normally exhibit a modest increase in the intracellular calcium levels after several days in culture under control conditions (red lines). In contrast, cells grown in media containing Aβ (blue lines) exhibit a much greater increase in intracellular [Ca²⁺]. The calcium values measured at day 3 are greater than at day 7. b The frequency histogram distribution for all neurons analyzed shows that the distribution of cells at day 1 and at day 7 was similar. Cells analyzed at day 7 represent the group of neurons most resistant to Aβ42