Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 6;15:35. doi: 10.1186/s12885-015-1035-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Hansen et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Detection of polyploidy. (A) SNP and copy number data across chromosome 17 from tumor sample 5. The top panel displays the copy number probe intensity calls and the calculated copy number segments (in color). The calculated segment (green line) has an intensity value of just over 2. The lower panel displays the calculated SNP allele ratios and shows that the entire chromosome 17 is in allelic balance. The vertical red line indicates the position of HER2. (B) SNP and copy number data across chromosome 14 from tumor sample 5. The enlargement of the red box shows that a segment (green line) is predicted with an intensity value of just under 2. However, a weak allelic imbalance (green arrows) suggests that the intensity value of just under 2 does not correspond to 2 DNA copies. Moreover, a deletion (~1.6) and an amplification (~2.2) only result in a modest copy number intensity change. Taken together, the data in (A) and (B) suggest that a segment with an intensity value of just over 2 and allelic balance must correspond to at least 4 copies of DNA. (C) Representative image of a 3D-rendered model of a confocal image stack of a section from tumor sample 5 hybridized with HER2 (red) and CEP17 (green) probes. The image extends 6 μm down into the z-axis, corresponding to ~60–70% of the nucleus diameter.