Table 1.
Primer pairs used for qPCR amplification
| ID v1.0 | Consensus annotation | Forward primer | Reverse primer | Amplicon | Slope |
|---|---|---|---|---|---|
| 90504 | Glycoside hydrolase family 7 protein | TTGGTTATGAGCTTGTGGGATGATTACTACGCCAACA | GGGAACTCCAGAGCTAGTAGAACAACTTCCTCGCTTAG | 126 | 3.42 |
| 46158 | Secreted short-chain dehydrogenase/reductase | GTGGAGCAGATTAGTGACACGGAAGC | CTACTGCAATACAGACCTCGACTCGCAAG | 178 | 3.84 |
| 86039 | Secreted NAD-dependent epimerase/dehydratase | GGTCAACGAGGAAACTCGCGCCACTAG | GTGACGGTATCATCGCGGCCAACG | 182 | 3.37 |
| 17705 | Small secreted cysteine rich | CAATTTTGCAAGAGTCCATTGGTTGTGTCAAC | GATTGTTGAGCTGAGTTACTGGCCGAAT | 144 | 3.44 |
| 19757 | Class II hydrophobin | GACTGCAAGACTCCCACTCAAGCCACCT | GGGAAGAGCATCCTGGCACAAAACAC | 134 | 3.49 |
| 42536 | Endoglucanase | GCTCATGATCTGGCTCGGAAAGTATGGA | GCTCATGATCTGGCTCGGAAAGTATGGAGGAGGGCGCCACAAAGCTATAAACTTG | 141 | 3.69 |
| 51095 | Polygalacturonase, glycoside hydrolase family 28 | GACGTATCAGGTATCACTCTATCATCTATCACAGGCTATGGTA | TAGCACTGGATAGAACACCGCCGCTTCC | 163 | 3.39 |
| 51211 | Acetyl xylan esterase | GGATACTCACAGGGCGGCCAAATC | GTTCCGACATTATATGACAGTCCGTGAATG | 175 | 3.87 |
| 54057 | Secreted short-chain dehydrogenase/reductase | GCACTGCATAAGGCCCTTCAGCTAGAG | CGCCTCGCCCACGGTATCAACG | 167 | 3.82 |
| 56652 | Xylanase, glycoside hydrolase family 10 | GCTTTACGCTTGGGATGTTGTCAATGAG | CCTTTAGTCTTGGCATAATTTGGGTCATC | 191 | 4.25 |
| 88010 | Beta tubulin | ACTTCAACGAGGCTTCTGGCAAC | CGGAACAGCTGGCCAAAGG | 108 | 3.36 |
The slope of the calibration line is given in the last column, measured from a dilution series of pooled cDNA samples, using ABI software (see Methods). The protein model for the small secreted protein (SSP) ID 17705 is ID 230434 in v2.0 of the database.