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. 2015 Feb 12;10(2):e0117546. doi: 10.1371/journal.pone.0117546

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© 2015 Yin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Localization of PCNA in the cytosolic and nuclear fractions of SH-SY5Y cells was examined by Western blot with antibodies against PCNA, Lamin A (nuclei marker), and aldose reductase (AR, cytosolic marker). (B) Localization of PCNA in SH-SY5Y cells was observed by confocal microscopy. Signal for aldose reductase served as cytosolic marker. (C) Effect to the localization of PCNA in SH-SY5Y cells by rotenone treatment was monitored by confocal microscopy with immunofluorescence using the PCNA antibody. DAPI was used as a nuclei stain.