Skip to main content
. 2015 Feb 12;9(2):e0003358. doi: 10.1371/journal.pntd.0003358

Figure 2. Inhibition of molting and reduction in transcription levels of RHBP and CAT in third instar nymphs.

Figure 2

Nymphs were fed blood with E. coli producing RHBP and CAT dsRNA. (A) Reduction of molting in third instar nymphs fed bacteria producing RHBP or CAT dsRNA as compared with nymphs fed blood without bacteria and with bacteria expressing ANT dsRNA (two biological replicates, n = 10 each). (B) Relative expression of RHBP in third instar nymphs fed bacteria producing RHBP dsRNA (two biological replicates, n = 3 each). (C) Relative expression of CAT in midguts of third instar nymphs fed with bacteria producing RHBP dsRNA (two biological replicates, n = 3 each). Asterisk indicates statistically different values compared with the control fed blood alone (T-test, P< 0.05).