Table 2.

PHA production of different phaC genes in recombinant C. necator PHBˉ4 ^a

Sample name	Dry cell weight (g/L)	PHA content (wt %)	PHA composition
C. necator H16 (Wild type)	4.1 ± 0.5	70 ± 5	P(3HB)
C. necator PHBˉ4(PHB-negative mutant)	1.2 ± 0	n/d	n/d
I-GG1	0.9 ± 0	n/d	n/d
I-GG12	0.9 ± 0	n/d	n/d
I-GG18	2.6 ± 0.1	60 ± 8	P(3HB)
I-GG18 (without promoter)	0.9 ± 0	n/d	n/d

^aCells were cultivated in MM medium at 30°C, 200 rpm for 48 hours with 20 gL⁻¹ of fructose as the sole carbon source.

n/d = not detected.