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. 2014 Nov 26;13:257. doi: 10.1186/1476-4598-13-257

Figure 1.

Figure 1

Down-regulation of SOX1 in NPC cell lines and tissues is associated with promoter hypermethylation. (A) Endogenous protein level (upper panel) and mRNA level (lower panel) of SOX1 were detected in NPC cell lines via WB and RT-PCR, respectively. (B) SOX1 transcripts of NPC tissues (T) and their corresponding adjacent non-tumor tissues (N) were determined via qRT-PCR and normalized using GAPDH expression. Data were analyzed via the ΔΔCt method and representative results from three samples (numbers 2, 3 and 23) are shown. Bar represents mean ± SD of three independent experiments (***p <0.001, Student’s t test). (C) Methylation status of NPC cell lines was determined by qMS-PCR. M, methylated SOX1; U, unmethylated SOX1. (D) NPC cell lines CNE2 and HONE1 were treated with or without 5 or 25 μM 5-Aza-CdR for 48 h. SOX1 transcripts were analyzed via qRT-PCR and normalized using GAPDH. Data were analyzed using the ΔΔCt method. Bar represents mean ± SD of three independent experiments (**p <0.01, ANOVA followed by the least significant difference test was used to make statistical comparisons).