Figure 6. Thy1⁺Sca-1^hi ILC3s are the main drivers of the intestinal pathogenesis.

(a) Protocol used for in vivo depletion of Thy1⁺ cells in newborn V23 mice. The pregnant mothers were administered intravenously with 1 mg rat anti-Thy1 mAb or 1 mg isotype control mAb at gestational days 15.5, 16.5, 17.5 and 18.5. After birth pups were injected i.p. daily with 100 μg anti-Thy1 mAb or 100 μg isotype control per mouse for 4 days. (b) Flow cytometric analysis of the Lin⁻Thy1⁺Sca-1^hi population in the small intestine after antibody injection. Dot plots show cells gated on CD45⁺Lin⁻. (c) Survival curves of V23 pups treated with anti-Thy1 (n = 12) or isotype (n = 12). ** P < 0.01 between groups by Log-rank test. (d) Flow cytometry plot showing the proportion of Lin⁻Thy1⁺Sca-1^hi cells in the small intestine of V23, V23Rorc(γt)^+/−, and V23Rorc(γt)^−/− mice at P0. Dot plots show cells gated on CD45⁺Lin⁻. (e) Survival curves of V23 (n = 15), V23Rorc(γt)^+/− (n = 18), and V23Rorc(γt)^−/− (n = 22) mice. *** P < 0.001 between V23Rorc(γt)^+/− /V23Rorc( γt)^−/− mice and V23 mice by Log-rank test. Data are representative of three independent experiments.