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. 2015 Jan 9;22(3):499–508. doi: 10.1038/cdd.2014.219

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Copyright © 2015 Macmillan Publishers Limited

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Identification of psd1Δ and psd2Δ as progeroid yeast strains. (a) The ROS-driven conversion of DHE to ethidium was measured at day three of chronological ageing in eleven yeast strains deficient for different enzymes involved in phospholipid synthesis. (b) The chronological ageing curve, which is based on the clonogenic survival, is shown for psd1Δ and psd2Δ in comparison with the wild type. (c) Phosphatidylethanolamine (PE) and phosphatidyl choline (PC) were quantified by HPLC-ELSD of lipid extracts from wild-type psd1Δ and psd2Δ cells at day three of chronological ageing