Fig. 2.

The concentration of irisflorentin for experiments was determined with a food clearance assay. Between 20 and 30 newly hatched L1 synchronized animals of N2, BZ555 or OW13 were incubated in E. coli (OD A₅₉₅ = 0.6) in a 96-well plate at 25°C containing different irisflorentin concentrations to a total volume of 60 μL. The OD of the plate was measured daily for 6 days. Note the lower OD (~0.6) in a well due to the decreased path length of the 60 μL final suspension compared to the 1 cm path length in a spectrophotometer. The OD of E. coli was recorded daily for each concentration of irisflorentin.