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. 2015 Feb 2;5(1):4. doi: 10.7603/s40681-015-0004-y

Fig. 6.

Fig. 6

Fig. 6

Irisflorentin increases proteasome activity by enhancing rpn-3 expression in the OW13 strain of C. elegans. (A) Chymotrypsin-like activity of the proteasome was monitored by Z-Gly-Gly-Leu-AMC digestion in a day 3 extract of adult animal containing equal amounts of total protein. The data represent the mean ± SD (n = 3). A hash (#) indicates significant differences between N2 and OW13 animals (P < 0.01); an asterisk (*) indicates significant differences between the OW13 control samples and the irisflorentin-treated OW13 samples (* P < 0.05, ** P < 0.01). (B) Irisflorentin raises the expression of rpn3 of the regulatory subunit of proteasome in the OW13 strain of C. elegans. Quantitative real-time RT-PCR experiments show the expression level of the Rpn subunit of the 26S proteasome using cDNAs isolated from OW13 control or irisflorentin-treated animals. The data represent the mean ± SD (n = 3). An asterisk (*) indicates significant differences between the OW13 control samples and the irisflorentintreated samples (** P < 0.01).