Fig. 1.

Transcriptional analysis of gene expression under temperature, acid and osmotic stress conditions using RT-PCR. Total RNA was isolated from exponential phase cultures of L. monocytogenes EGD strain grown in BHI at 37 °C (1) and exposed to 42 °C (2) or pH 5 (3) or 0.5 M KCl (4) for 90 min in each case. All RT-PCRs were performed three times from three separate RNA preparations. In all cases, control PCR were performed to ensure the complete removal of DNA from RNA preparations prior to reverse transcription