Fig. 4.

Effect of C-6-H on hla expression in saeRS mutant strains. S. aureus SH1000 wt, SH1000 saeS∷Tn551 and SH1000 saeR∷Tn551 were grown in TSB^−Fe until OD₆₀₀ 0.5. 8 µg/ml C-6-H was added to the cultures, and cells were incubated for 10 (striped bars) or 60 (filled bars) min. Total mRNA was isolated, and an qRT-PCR experiment was performed determining hla expression. Red line shows significant change of at least 0.5-fold. The samples were measured in triplicate, and qRT-PCR experiment was carried out three times