Table 4.
Growth phase associated changes in extracellular protein profile
| ORF N315 | Protein | Gene product | OD 1.0 versus 16 h | Spot ID |
|---|---|---|---|---|
| SA0009 | SerS | Seryl-tRNA synthetase | 0.65 | 331 |
| SA0091 | Plc | 1-Phosphatidylinositol phosphodiesterase precurosr | 2.29 | 563 |
| SA0091 | Plc | 1-Phosphatidylinositol phosphodiesterase precurosr | 4.83 | 565 |
| SA0091 | Plc | 1-Phosphatidylinositol phosphodiesterase precurosr | 38.19 | 571 |
| SA0128 | SodM (SodA1) | Superoxide dismutase | 1.60 | 698 |
| SA0131 | Pnp (DeoD1) | Purine nucleoside phosphorylase | 1.11 | 654 |
| SA0131 | Pnp (DeoD1) | Purine nucleoside phosphorylase | 0.06 | 187 |
| SA0162 | AldA | Aldehyde dehydrogenase homologue | 0.27 | 294 |
| SA0182 | Hypothetical protein, similar to indole-3-pyruvate decarboxylas | 1.12 | 280 | |
| SA0265 | LytM | Peptidoglycan hydrolase | 0.15 | 454 |
| SA0265 | LytM | Peptidoglycan hydrolase | 0.13 | 460 |
| SA0309 | Geh | Glycerol ester hydrolase | 2.29 | 170 |
| SA0309 | Geh | Glycerol ester hydrolase | 3.08 | 171 |
| SA0309 | Geh | Glycerol ester hydrolase | 2.84 | 172 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.81 | 175 |
| SA0309 | Geh | Glycerol ester hydrolase | 4.03 | 177 |
| SA0309 | Geh | Glycerol ester hydrolase | 9.64 | 199 |
| SA0309 | Geh | Glycerol ester hydrolase | 3.28 | 212 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.76 | 224 |
| SA0309 | Geh | Glycerol ester hydrolase | 21.07 | 229 |
| SA0309 | Geh | Glycerol ester hydrolase | 5.23 | 272 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.81 | 282 |
| SA0309 | Geh | Glycerol ester hydrolase | 0.83 | 421 |
| SA0309 | Geh | Glycerol ester hydrolase | 2.75 | 433 |
| SA0309 | Geh | Glycerol ester hydrolase | 0.57 | 439 |
| SA0309 | Geh | Glycerol ester hydrolase | 3.00 | 443 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.20 | 451 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.90 | 176 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.17 | 217 |
| SA0309 | Geh | Glycerol ester hydrolase | 3.97 | 221 |
| SA0309 | Geh | Glycerol ester hydrolase | 0.68 | 238 |
| SA0309 | Geh | Glycerol ester hydrolase | 2.99 | 248 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.32 | 259 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.97 | 293 |
| SA0309 | Geh | Glycerol ester hydrolase | 3.40 | 418 |
| SA0309 | Geh | Glycerol ester hydrolase | 8.89 | 424 |
| SA0309 | Geh | Glycerol ester hydrolase | 5.58 | 435 |
| SA0309 | Geh | Glycerol ester hydrolase | 1.15 | 436 |
| SA0366 | AhpC | Alkyl hydroperoxide reductase subunit C | 3.04 | 712 |
| SA0375 | GuaB | Inositol-monophosphate dehydrogenase | 0.34 | 308 |
| SA0376 | GuaA | GMP synthase | 2.00 | 274 |
| SA0382 | Set6 | Superantigen-like protein | 0.03 | 706 |
| SA0482 | Putative ATP: guanido phosphotransferase SA0482 | 0.76 | 366 | |
| SA0486 | GltX | Glutamyl-tRNA synthetase | 1.21 | 289 |
| SA0486 | GltX | Glutamyl-tRNA synthetase | 0.41 | 302 |
| SA0488 | CysS | Cysteinyl-tRNA synthetase | 0.64 | 309 |
| SA0505 | FusA | Elongation factor G | 0.14 | 162 |
| SA0506 | Tuf | Elongation factor Tu | 0.69 | 371 |
| SA0506 | Tuf | Elongation factor Tu | 0.63 | 396 |
| SA0544 | Putative haem peroxidase | 0.21 | 618 | |
| SA0587 | Lipoprotein, streptococcal adhesin PsaA homologue | 1.63 | 561 | |
| SA0587 | Lipoprotein, streptococcal adhesin PsaA homologue | 0.22 | 610 | |
| SA0620 | Secretory antigen SsaA homologue | 0.28 | 626 | |
| SA0674 | Glycerol phosphate lipoteichoic acid synthase | 0.87 | 336 | |
| SA0674 | Glycerol phosphate lipoteichoic acid synthase | 0.53 | 343 | |
| SA0674 | Glycerol phosphate lipoteichoic acid synthase | 1.15 | 344 | |
| SA0674 | Glycerol phosphate lipoteichoic acid synthase | 0.70 | 346 | |
| SA0674 | Glycerol phosphate lipoteichoic acid synthase | 0.50 | 353 | |
| SA0686 | NrdE | Ribonucleotide-diphosphate reductase subunit alpha | 0.51 | 189 |
| SA0719 | TrxB | Thioredoxin reductase | 1.82 | 508 |
| SA0727 | Gap | Glyceraldehyde-3-phosphate dehydrogenase | 0.54 | 447 |
| SA0728 | Pgk | Phosphoglycerate kinase | 0.57 | 409 |
| SA0731 | Eno | Phosphopyruvate hydratase | 0.55 | 384 |
| SA0732 | ClpP | ClpP | 1.07 | 729 |
| SA0775 | Hypothetical protein | 0.52 | 296 | |
| SA0787 | IS1181 transposase | 0.37 | 242 | |
| SA0802 | NADH dehydrogenase-like protein SA0802 | 0.94 | 411 | |
| SA0820 | GlpQ | Glycerophosphoryl diester phosphodiesterase | 2.31 | 569 |
| SA0820 | GlpQ | Glycerophosphoryl diester phosphodiesterase | 1.72 | 570 |
| SA0823 | Pgi | Glucose-6-phosphate isomerase | 1.10 | 378 |
| SA0829 | Hypothetical protein | 0.16 | 573 | |
| SA0831 | Cdr | Coenzyme A disulphide reductase | 2.08 | 349 |
| SA0842 | FabH | FabH, 3-oxoacyl-(acyl carrier protein) synthase homologue | 1.04 | 489 |
| SA0843 | Fab (FabF) | 3-oxoacyl-synthase | 0.94 | 362 |
| SA0900 | SspB1 | Cysteine protease precursor SspB | 1.72 | 427 |
| SA0900 | SspB1 | Cysteine protease precursor SspB | 1.25 | 432 |
| SA0900 | SspB1 | Cysteine protease precursor SspB | 2.53 | 468 |
| SA0900 | SspB1 | Cysteine protease precursor SspB | 1.51 | 825 |
| SA0901 | SspA | V8 protease | 0.77 | 474 |
| SA0901 | SspA | V8 protease | 1.26 | 478 |
| SA0901 | SspA | V8 protease | 1.30 | 483 |
| SA0901 | SspA | V8 protease | 1.73 | 486 |
| SA0901 | SspA | V8 protease | 0.39 | 507 |
| SA0901 | SspA | V8 protease | 1.47 | 511 |
| SA0901 | SspA | V8 protease | 1.37 | 490 |
| SA0904 | Atl | ATL autolysin transcription regulator | 0.28 | 163 |
| SA0908 | Hypothetical protein | 1.80 | 417 | |
| SA0908 | Hypothetical protein | 1.90 | 419 | |
| SA0935 | PtsI | Phosphoenolpyruvate-protein phosphatase | 0.09 | 244 |
| SA0939 | Hypothetical protein | 0.12 | 676 | |
| SA0945 | PdhC | Branched-chain alpha-keto acid dehydrogenase subunit E2 | 0.47 | 192 |
| SA0946 | PdhD | Dihydrolipoamide dehydrogenase | 1.04 | 287 |
| SA1007 | Hla | Alpha-haemolysin | 5.28 | 531 |
| SA1007 | Hla | Alpha-haemolysin | 2.71 | 536 |
| SA1007 | Hla | Alpha-haemolysin | 5.04 | 539 |
| SA1007 | Hla | Alpha-haemolysin | 4.60 | 541 |
| SA1007 | Hla | Alpha-haemolysin | 1.92 | 651 |
| SA1036 | IleS | Isoleucyl-tRNA synthetase | 0.41 | 132 |
| SA1098 | CodY | Transcriptional repressor CodY | 2.87 | 617 |
| SA1099 | RpsB | 30S ribosomal protein S2 | 0.55 | 514 |
| SA1100 | Tsf | Elongation factor Ts | 2.23 | 459 |
| SA1100 | Tsf | Elongation factor Ts | 1.58 | 470 |
| SA1128 | RecA | Recombinase A | 0.79 | 389 |
| SA1150 | GlnA | Glutamine–ammonia ligase | 1.70 | 323 |
| SA1170 | KatA | Catalase | 1.87 | 263 |
| SA1177 | Tkt | Transketolase | 0.40 | 197 |
| SA1177 | Tkt | Transketolase | 1.31 | 201 |
| SA1177 | Tkt | Transketolase | 3.11 | 401 |
| SA1533 | AckA | Acetate kinase homologue | 0.40 | 393 |
| SA1184 | CitB (AcnA) | Aconitate hydratase | 0.23 | 128 |
| SA1216 | PepF | Hypothetical protein, similar to oligoendopeptidase | 33.50 | 215 |
| SA1283 | Pbp2 | PBP2 | 0.57 | 220 |
| SA1308 | RpsA | 30S ribosomal protein S1 | 0.43 | 363 |
| SA1336 | Glucose-6-phosphate 1-dehydrogenase | 1.50 | 250 | |
| SA1342 | Gnd | 6-Phosphogluconate dehydrogenase | 2.22 | 391 |
| SA1342 | Gnd | 6-Phosphogluconate dehydrogenase | 2.20 | 400 |
| SA1359 | Efp | Elongation factor P | 0.40 | 560 |
| SA1409 | DnaK | Molecular chaperone DnaK | 0.69 | 226 |
| SA1409 | DnaK | Molecular chaperone DnaK | 2.15 | 546 |
| SA1499 | Tig | Trigger factor | 0.10 | 231 |
| SA1520 | PykA | Pyruvate kinase | 0.68 | 203 |
| SA1529 | Metal-dependent hydrolase | 6.88 | 669 | |
| SA1553 | Fhs | Formate-tetrahydrofolate ligase | 2.57 | 273 |
| SA1553 | Fhs | Formate-tetrahydrofolate ligase | 1.16 | 277 |
| SA1579 | LeuS | Leucyl-tRNA synthetase | 2.68 | 143 |
| SA1599 | Tal | Hypothetical protein, similar to transaldolase | 1.17 | 659 |
| SA1609 | PckA | Phosphoenolpyruvate carboxykinase | 2.05 | 279 |
| SA1627 | SplF | Serine protease SplE, putative | 2.93 | 667 |
| SA1627 | SplF | Serine protease SplE, putative | 7.46 | 660 |
| SA1627 | SplF | Serine protease SplE, putative | 7.88 | 670 |
| SA1628 | SplD | Serine protease SplD | 4.68 | 666 |
| SA1629 | SplC | Serine protease SplC | 4.43 | 656 |
| SA1629 | SplC | Serine protease SplC | 1.41 | 657 |
| SA1630 | SplB | Serine protease SplB | 6.64 | 646 |
| SA1631 | SplA | Serine protease SplA | 4.55 | 642 |
| SA1631 | SplA | Serine protease SplA | 2.01 | 647 |
| SA1637 | LukD | Leukotoxin, LukD | 1.22 | 487 |
| SA1653 | TRAP | Signal transduction protein TRAP | 6.36 | 914 |
| SA1695 | AmpS | Aminopeptidase ampS | 1.34 | 397 |
| SA1709 | Ferritin | 0.32 | 910 | |
| SA1725 | SspB2 | Staphopain, cysteine proteinase | 1.76 | 725 |
| SA1725 | SspB2 | Staphopain, cysteine proteinase | 6.01 | 754 |
| SA1811 | Hlb | Beta-haemolsysin | 1.28 | 505 |
| SA1811 | Hlb | Beta-haemolsysin | 1.02 | 509 |
| SA1811 | Hlb | Beta-haemolsysin | 0.38 | 515 |
| SA1811 | Hlb | Beta-haemolsysin | 5.31 | 519 |
| SA1811 | Hlb | Beta-haemolsysin | 2.31 | 520 |
| SA1811 | Hlb | Beta-haemolsysin | 0.29 | 522 |
| SA1811 | Hlb | Beta-haemolsysin | 0.65 | 574 |
| SA1812 | Uncharacterized leukocidin-like protein 1 precursor | 1.64 | 499 | |
| SA1812 | Uncharacterized leukocidin-like protein 1 precursor | 2.19 | 500 | |
| SA1812 | Uncharacterized leukocidin-like protein 1 precursor | 1.21 | 502 | |
| SA1813 | Uncharacterized leukocidin-like protein 2 precursor | 0.72 | 494 | |
| SA182 | SodA (SodA2) | Superoxide dismutase SodA | 1.53 | 697 |
| SA1836 | GroEL | Chaperonin GroEL | 0.37 | 267 |
| SA1898 | Hypothetical protein, similar to SceD precursor | 0.27 | 552 | |
| SA1905 | AtpD | F0F1 ATP synthase subunit beta | 0.28 | 383 |
| SA1915 | GlyA | Serine hydroxymethyltransferase | 1.24 | 364 |
| SA1915 | GlyA | Serine hydroxymethyltransferase | 0.90 | 367 |
| SA1927 | FbaA | Fructose-bisphosphate aldolase | 0.61 | 530 |
| SA1959 | GlmS | Glucosamine-fructose-6-phosphate transferase | 1.12 | 218 |
| SA1984 | Asp23 | Alkaline shock protein 23 | 1.66 | 827 |
| SA2003 | HysA | Hyaluronate lyase precursor | 0.30 | 156 |
| SA2093 | SsaA | Secretory antigen precursor SsaA homologue | 0.09 | 592 |
| SA2093 | SsaA | Secretory antigen precursor SsaA homologue | 0.10 | 593 |
| SA2097 | Hypothetical protein, similar to secretory antigen precursor SsaA | 0.24 | 860 | |
| SA2204 | GpmA | Phosphoglycerate mutase, pgm homologue | 3.01 | 583 |
| SA2204 | GpmA | Phosphoglycerate mutase, pgm homologue | 1.36 | 585 |
| SA2206 | Sbi | IgG-binding protein SBI | 0.29 | 387 |
| SA2208 | HlgC | Gamma-haemolysin component C | 3.73 | 535 |
| SA2209 | HlgB | Gamma-haemolysin component B | 2.47 | 497 |
| SA2334 | MmvaS | 3-Hydroxy-3-methylglutaryl CoA synthase | 0.68 | 434 |
| SA2336 | ClpL | ATP-dependent Clp proteinase chain clpL | 0.35 | 210 |
| SA2356 | IsaA | Immunodominant antigen A | 0.23 | 616 |
| SA2356 | IsaA | Immunodominant antigen A | 0.22 | 635 |
| SA2356 | IsaA | Immunodominant antigen A | 2.08 | 747 |
| SA2356 | IsaA | Immunodominant antigen A | 1.43 | 822 |
| SA2356 | IsaA | Immunodominant antigen A | 0.24 | 908 |
| SA2430 | Aur | Zinc metalloproteinase aureolysin | 0.21 | 471 |
| SA2430 | Aur | Zinc metalloproteinase aureolysin | 0.79 | 496 |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.36 | 191 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.19 | 193 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.16 | 195 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.11 | 200 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.88 | 223 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 1.11 | 235 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 1.15 | 236 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.10 | 245 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.78 | 269 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.41 | 422 |
Table of all identified protein spots from the extracellular fraction. Data for proteins with a spot vol. ratio of ≥2 and ≤0.5 are shown. All proteins had a significance level of 0.05 or less (T test 5 % cut-off). Proteins highlighted in italics are significantly changed in the two phases of growth