Table 5.
Growth phase-dependent changes in extracellular protein profile
| ORF N315 | Protein | Gene product | OD 1.0 versus 16 h | Spot ID |
|---|---|---|---|---|
| SA0265 | LytM | Peptidoglycan hydrolase | 0.15 | 454 |
| SA0265 | LytM | Peptidoglycan hydrolase | 0.13 | 460 |
| SA0309 | Geh | Glycerol ester hydrolase | 3.08 | 171 |
| SA0309 | Geh | Glycerol ester hydrolase | 9.64 | 199 |
| SA0309 | Geh | Glycerol ester hydrolase | 21.07 | 229 |
| SA0309 | Geh | Glycerol ester hydrolase | 8.89 | 424 |
| SA0375 | GuaB | Inositol-monophosphate dehydrogenase | 0.34 | 308 |
| SA0393 | Set15 | Superantigen-like protein | 0.12 | 676 |
| SA0505 | FusA | Elongation factor G | 0.14 | 162 |
| SA0544 | Putative haem peroxidase | 0.21 | 618 | |
| SA0935 | PtsI | Phosphoenolpyruvate-protein phosphatase | 0.09 | 244 |
| SA0945 | PdhC | Branched-chain alpha-keto acid dehydrogenase subunit E2 | 0.47 | 192 |
| SA1007 | Hla | Alpha-haemolysin | 5.28 | 531 |
| SA1007 | Hla | Alpha-haemolysin | 4.60 | 541 |
| SA1177 | Tkt | Transketolase | 0.40 | 197 |
| SA1499 | Tig | Trigger factor | 0.10 | 231 |
| SA1627 | SplF | Serine protease SplE, putative | 7.88 | 670 |
| SA1628 | SplD | Serine protease SplD | 4.68 | 666 |
| SA1630 | SplB | Serine protease SplB | 6.64 | 646 |
| SA1725 | Staphopain, cysteine proteinase | 6.01 | 754 | |
| SA1898 | Hypothetical protein, similar to SceD precursor | 0.27 | 552 | |
| SA2093 | SsaA | Secretory antigen precursor SsaA homologue | 0.09 | 592 |
| SA2093 | SsaA | Secretory antigen precursor SsaA homologue | 0.10 | 593 |
| SA2204 | GpmA | Phosphoglycerate mutase, pgm homologue | 3.01 | 583 |
| SA2208 | HlgC | Gamma-haemolysin component C | 3.73 | 535 |
| SA2209 | HlgB | Gamma-haemolysin component B | 2.47 | 497 |
| SA2356 | IsaA | Immunodominant antigen A | 0.22 | 635 |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.16 | 195 | |
| SA2437 | Hypothetical protein, similar to autolysin precursor | 0.41 | 422 |
Comparison of the pattern of extracellular protein expression in exponential phase (OD600 1.0) and stationary phase of S. aureus. Data for proteins with a spot vol. ratio of ≥2 and ≤0.5 are shown. All genes had a significance level of 0.05 or less (T test 5 % cut-off)